Dependence of the Photosynthetic Quantum Yield on Phytoplankton Light Absorption: Equations for Assessing Primary Production in the Black Sea

T. Ya. Churilova1, ✉, V. V. Suslin2, H. M. Sosik3

1 A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Sevastopol, Russian Federation

2 Marine Hydrophysical Institute of RAS, Sevastopol, Russian Federation

3 Woods Hole Oceanographic Institution, Woods Hole, USA

✉ e-mail: tanya.churilova@ibss-ras.ru

Abstract

Purpose. Investigations were performed during a scientific cruise to characterize hydrophysical properties, chlorophyll a concentration, photosynthesis-irradiance curves, spectral light absorption coefficients by phytoplankton, and spectral quantum downwelling irradiance. From these results, the dependence of the photosynthetic quantum yield upon environmental factors was studied with the purpose of adapting an algorithm developed for the Baltic Sea so that it can be applied for the Black Sea.

Methods and Results. Complex hydrophysical and biological studies were carried out at several depths within the euphotic zone. Spectral bio-optical parameters were measured in accordance with the latest NASA protocols (2018). Experiments to determine the photosynthesis-light relationship were performed under temperature and light conditions similar to those in situ. The quantum yield of photosynthesis was calculated from parameters of photosynthesis-light curves (photosynthesis efficiency, light saturation parameter) and the spectral light absorption coefficients by phytoplankton pigments. It was found out that the main photosynthetic characteristics of phytoplankton, including the maximum photosynthetic quantum yield and the fraction of phytoplankton absorption associated with photoprotective accessory pigments, varied with depth within the euphotic zone, due to phytoplankton acclimation to environment factors during the period of seasonal stratification. The relationship between the photosynthetic quantum yield and the quanta absorbed by photosynthetically active phytoplankton pigments was revealed. The results of this research made it possible to build on the approach developed for other regions and modify the equation for calculating the quantum yield to apply specifically for environmental conditions in the Black Sea.

Conclusions. For the first time, comprehensive studies carried out in the Black Sea, including measurements of the photosynthesis-light dependence, spectral light absorption coefficients by phytoplankton and spectral downwelling irradiance as a function of optical depths within the euphotic zone, made it possible to reveal the equation for calculating photosynthetic quantum yield. This new equation can be applied for calculating primary production of the Black Sea using a spectral approach, based both on the results of in situ measurements and remote sensing data.

Keywords

phytoplankton, pigments, photosynthesis, quantum yield, light absorption, photosynthesis, Black Sea

Acknowledgements

The research was carried out within the framework of the state task on theme AAAA-А19-119061190081-9 and also at partial support of the RFBR grant 18-45-920070. HMS acknowledges support of the Simons Foundation (award 561126).

Original russian text

Original Russian Text © T. Ya. Churilova, V. V. Suslin, H. M. Sosik, 2021, published in MORSKOY GIDROFIZICHESKIY ZHURNAL, Vol. 37, Iss. 1, pp. 73-84 (2021)

For citation

Churilova, T.Ya., Suslin, V.V. and Sosik, H.M., 2021. Dependence of the Photosynthetic Quantum Yield on Phytoplankton Light Absorption: Equations for Assessing Primary Production in the Black Sea. Physical Oceanography, 28(1), pp. 67-77. doi:10.22449/1573-160X-2021-1-67-77

DOI

10.22449/1573-160X-2021-1-67-77

References

  1. Geider, R.J., Delucia, E.H., Falkowski, P.G., Finzi, A.C., Grime, J.P., Grace, J., Kana, T.M., La Roche, J., Long, S.P. [et al.], 2001. Primary Productivity of Planet Earth: Biological Determinants and Physical Constraints in Terrestrial and Aquatic Habitats. Global Change Biology, 7(8), pp. 849-882. https://doi.org/10.1046/j.1365-2486.2001.00448.x
  2. Falkowski, P.G. and Raven, J.A., 2007. Aquatic Photosynthesis. Princeton: Princeton University Press, 488 p. doi:10.2307/j.ctt4cgbxs
  3. Williams, P.J. le B., Thomas, D.N. and Reynolds, C.S., Eds., 2002. Phytoplankton Productivity: Carbon Assimilation in Marine and Freshwater Ecosystems. Garsington: Blackwell Science Ltd, 400 p. doi:10.1002/9780470995204
  4. Churilova, T.Ya., Suslin, V.V., Krivenko, O.V., Efimova, T.V. and Moiseeva, N.A., 2016. Spectral Approach to Assessment of Phytoplankton Photosynthesis Rate in the Black Sea Based on Satellite Information: Methodological Aspects of the Regional Model Development. Journal of Siberian Federal University. Biology, 9(4), pp. 367-384. https://doi.org/10.17516/1997-1389-2016-9-4-367-384 (in Russian).
  5. Churilova, T., Suslin, V., Krivenko, O., Efimova, T., Moiseeva, N., Mukhanov, V. and Smirnova, L., 2017. Light Absorption by Phytoplankton in the Upper Mixed Layer of the Black Sea: Seasonality and Parameterization. Frontiers in Marine Science, 4, 90. https://doi.org/10.3389/fmars.2017.00090
  6. Churilova, T., Suslin, V., Sosik, H.M., Efimova, T., Moiseeva, N., Moncheva, S., Mukhanov, V., Rylkova, O. and Krivenko, O., 2019. Phytoplankton Light Absorption in the Deep Chlorophyll Maximum Layer of the Black Sea. European Journal of Remote Sensing, 52(suppl. 1), pp. 123-136. https://doi.org/10.1080/22797254.2018.1533389
  7. Smyth, T.J., Tilstone, G.H. and Groom, S.B., 2005. Integration of Radiative Transfer into Satellite Models of Ocean Primary Production. Journal of Geophysical Research: Oceans, 110(C10), C10014. https://doi.org/10.1029/2004JC002784
  8. Woźniak, B., Ficek, D., Ostrowska, M., Majchrowski, R. and Dera, J., 2007. Quantum Yield of Photosynthesis in the Baltic: a New Mathematical Expression for Remote Sensing Applications. Oceanologia, 49(4), pp. 527-542. Available at: http://www.iopan.gda.pl/oceanologia/494wozni.pdf [Accessed: 10 January 2021].
  9. Churilova, T.Y., 2001. Light Absorption by Phytoplankton and Detritus in the Black Sea in Spring. Oceanology, 41(5), pp. 687-695.
  10. Yentsch, C.S., 1962. Measurement of Visible Light Absorption by Particulate Matter in the Ocean. Limnology and Oceanography, 7(2), pp. 207-217. https://doi.org/10.4319/lo.1962.7.2.0207
  11. Mitchell, B. and Kiefer, D.A., 1988. Chlorophyll a Specific Absorption and Fluorescence Excitation Spectra for Light-Limited Phytoplankton. Deep Sea Research Part A. Oceanographic Research Papers, 35(5), pp. 639-663. https://doi.org/10.1016/0198-0149(88)90024-6
  12. Neeley, A.R. and Mannino, A., Eds., 2018. IOCCG Ocean Optics and Biogeochemistry Protocols for Satellite Ocean Colour Sensor Validation. Volume 1.0 : Inherent Optical Property Measurements and Protocols: Absorption Coefficient. Dartmouth, NS, Canada: IOCCG, 78 p. http://dx.doi.org/10.25607/OBP-119
  13. Mitchell, B.G., 1990. Algorithms for Determining the Absorption Coefficient for Aquatic Particulates Using the Quantitative Filter Technique. Proceedings of SPIE: Ocean Optics X, 1302, pp. 137-148. https://doi.org/10.1117/12.21440
  14. Kishino, M., Takahashi, M., Okami, N. and Ichimura, S., 1985. Estimation of the Spectral Absorption Coefficients of Phytoplankton in the Sea. Bulletin of Marine Science, 37(2), pp. 634-642.
  15. Finenko, Z.Z., Churilova, T.Ya., Sosik, H.M. and Basturk, O., 2002. Variability of Photosynthetic Parameters of Surface Phytoplankton in the Black Sea. Oceanology, 42(1), pp. 53-67.
  16. Platt, T., Harrison, W.G., Irwin, B., Horne, E.P. and Gallegos, C.L., 1982. Photosynthesis and Photoadaptation of Marine Phytoplankton in the Arctic. Deep Sea Research Part A. Oceanographic Research Papers, 29(10), pp. 1159-1170. https://doi.org/10.1016/0198-0149(82)90087-5
  17. Bidigare, R.R., Prézelin, B.B. and Smith, R.C., 1992. Bio-Optical Models and the Problems of Scaling. In: P. G. Falkowski, A. D. Woodhead and K. Vivirito, Eds., 1992. Primary Productivity and Biogeochemical Cycles in the Sea. Boston, MA: Springer, pp. 175-212. https://doi.org/10.1007/978-1-4899-0762-2_11
  18. Marra, J., Trees, C.C., Bidigare, R.R. and Barber, R.T., 2000. Pigment Absorption and Quantum Yields in the Arabian Sea. Deep Sea Research Part II: Topical Studies in Oceanography, 47(7–8), pp. 1279-1299. https://doi.org/10.1016/S0967-0645(99)00144-7
  19. Kirk, J.T.O., 2011. Light and Photosynthesis in Aquatic Ecosystems. 3d ed. Cambridge: Cambridge University Press, 662 p. https://doi.org/10.1017/CBO9781139168212
  20. Babin, M., Morel, A., Claustre, H., Bricaud, A., Kolber, Z. and Falkowski, P.G., 1996. Nitrogen- and Irradiance-Dependent Variations of the Maximum Quantum Yield of Carbon Fixation in Eutrophic, Mesotrophic and Oligotrophic Marine Systems. Deep-Sea Research Part I: Oceanographic Research Papers, 43(8), pp. 1241-1272. https://doi.org/10.1016/0967-0637(96)00058-1
  21. Finenko, Z.Z., Churilova, T.Y. and Sosik, H.M., 2004. Vertical Distribution of Phytoplankton Photosynthetic Characteristics in the Black Sea. Oceanology, 44(2), pp. 205-218.
  22. Churilova, T., Suslin, V., Moiseeva, N. and Efimova, T., 2018. Dissolved and Suspended Matter Variability in Coastal Waters: Photosynthetic Available Light. Proceedings of SPIE: 24th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 10833, 1083365. https://doi.org/10.1117/12.2504637
  23. Churilova, T., Finenko, Z. and Tugrul, S., 2008. Light Absorption and Maximum Quantum Yield of Photosynthesis during Autumn Phytoplankton Bloom in the Western Black Sea. Morskoj Ehkologicheskij Zhurnal = Marine Ekological Journal, 7(3), pp. 75-86. Available at: https://repository.marine-research.org/bitstream/299011/975/1/MEJ_Churilova_et_al_2007_corrected.pdf [Accessed: 20 December 2020] (in Russian).
  24. MacIntyre, H.L., Kana, T.M., Anning, T. and Geider, R.J., 2002. Photoacclimation of Photosynthesis Irradiance Response Curves and Photosynthetic Pigments in Microalgae and Cyanobacteria. Journal of Phycology, 38(1), pp. 17-38. https://doi.org/10.1046/j.1529-8817.2002.00094.x
  25. Churilova, T.Ya., Finenko, Z.Z. and Akimov, A.I., 2008. Pigments of Microalgae. In: Yu. N.Tokarev, Z. Z. Finenko and N. V. Shadrin, Eds., 2008. The Black Sea Microalgae: Problems of Biodiversity Preservation and Biotechnological Usage. Sevastopol: ECOSI-Gidrofizika, pp. 301-319. Available at: http://repository.marine-research.org/handle/299011/5521 [Accessed: 20 December 2020] (in Russian).
  26. Woźniak, B., Dera, J., Ficek, D., Ostrowska, M. and Majchrowski, R., 2002. Dependence of the Photosynthesis Quantum Yield in Oceans on Environmental Factors. Oceanologia, 44(4), pp. 439-459. Available at: https://www.iopan.pl/oceanologia/444wozni.pdf [Accessed: 20 December 2020].
  27. Ficek, D., 2001. Modelling the Quantum Yield of Photosynthesis in Various Marine Systems. Sopot: Institute of Oceanology PAS, 224 p.

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